The Cytochromes of Prototheca zopfii

Identification of Prototheca zopfii from Bovine Mastitis

BACKGROUND The aim of this study was identification of the epidemiology of Prototheca zopfii species from the milk samples of dairy cattle in Isfahan, central Iran. METHODS Milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in Isfahan. The samples were cultured in Prototheca Isolation Medium (PIM) and Sabouraud's dextrose agar. All P. zopfii isolates were ide...

Prototheca zopfii mastitis in a herd of dairy cows.

Mastitis caused by the colourless alga Prototheca zopfii was diagnosed in 17 of 120 cows in a dairy herd. Infection occurred in animals varying from 3-14 years old and was present in one to four quarters of each cow. Nine cases were associated with clinical mastitis characterised by the presence in milk of flakes or small clots. Somatic cell counts consistent with subclinical mastitis (>500 x 1...

Inhibition of Respiration in Prototheca zopfii by Light.

Irradiation of cells of Prototheca zopfii with blue light inhibited the respiratory capacity of the cells. The inhibition of respiration was correlated with a photodestruction of cytochrome c(551), cytochrome b(559), and cytochrome a(3). Cytochrome c(549), cytochrome b(555), and cytochrome b(564) were unaffected by the irradiation treatment. The alpha-band of reduced cytochrome a was shifted fr...

The metabolism of acetate in the colourless alga, Prototheca zopfii.

Studies on the Metabolism of the Colorless Alga Prototheca Zopfii

The metabolism of Prototheca zopfii was investigated in an attempt to establish the specific function of its growth factor, thiamin. A study of the oxidative decomposition of various substrates by this organism demonstrated that the addition of catalytic amounts of thiamin to vitamin-deficient cells causes a pronounced stimulation in the rate of oxygen utilization during the degradation of cert...